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Fully Automated On-line SPE Coupled to LC MS/MS for Rapid, Sensitive, and Simultaneous Analysis of Multiple PPCPs in Water
Oral Presentation
Prepared by T. Anumol1, S. Mohsin2, S. Merel3, S. Snyder4
1 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
2 - Agilent Technologies, , Schaumburg, IL,
3 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
4 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
Contact Information: tanumol@email.arizona.edu; 412-656-0490
ABSTRACT
With the presence of Pharmaceuticals and Personal Care Products (PPCPs) in water now reported frequently and the synergistic effects of low doses largely unknown, the need for sensitive and rapid analytical measurement of these compounds is paramount. The traditional method to achieve low detection limits has been to use off-line concentration techniques like solid-phase extraction (SPE) or continuous liquid-liquid extraction (CLLE). This method requires large sample volume, is time consuming and labor-intensive. With the increasing sensitivity of new analytical instruments, Large Volume Injection (LVI) has been mooted as a possible solution; this technique though adversely affects the life of an analytical column while failing to remove possible interference in the matrix due to a lack of sample clean-up. Trace enrichment or on-line SPE eliminates all these difficulties while providing sample clean-up and reduces interferences which LVI cannot do. Further, it can be completely automated to reduce labor while increasing reproducibility of results. In this study, a single automated analytical method for the analysis of over 25 PPCPs (both in ESI- and ESI+) using on-line SPE coupled to a tandem mass spectrometer will be shown. Critical parameters like sample loading volume, extraction cartridge material and elution solvents will also be discussed. The results were compared to off-line SPE which showed comparable results while offering significant time, labor and solvent savings. Further, the online method significantly reduced ion suppression in complex matrixes compared to off-line SPEand LVI. The method was also run on several different water matrixes including surface water, ground water and wastewater, always producing accurate and reproducible results.
Oral Presentation
Prepared by T. Anumol1, S. Mohsin2, S. Merel3, S. Snyder4
1 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
2 - Agilent Technologies, , Schaumburg, IL,
3 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
4 - University of Arizona, 1657 E Helen Street, BIO5 Institute, Office: 0400A22, Tucson, AZ, 85719
Contact Information: tanumol@email.arizona.edu; 412-656-0490
ABSTRACT
With the presence of Pharmaceuticals and Personal Care Products (PPCPs) in water now reported frequently and the synergistic effects of low doses largely unknown, the need for sensitive and rapid analytical measurement of these compounds is paramount. The traditional method to achieve low detection limits has been to use off-line concentration techniques like solid-phase extraction (SPE) or continuous liquid-liquid extraction (CLLE). This method requires large sample volume, is time consuming and labor-intensive. With the increasing sensitivity of new analytical instruments, Large Volume Injection (LVI) has been mooted as a possible solution; this technique though adversely affects the life of an analytical column while failing to remove possible interference in the matrix due to a lack of sample clean-up. Trace enrichment or on-line SPE eliminates all these difficulties while providing sample clean-up and reduces interferences which LVI cannot do. Further, it can be completely automated to reduce labor while increasing reproducibility of results. In this study, a single automated analytical method for the analysis of over 25 PPCPs (both in ESI- and ESI+) using on-line SPE coupled to a tandem mass spectrometer will be shown. Critical parameters like sample loading volume, extraction cartridge material and elution solvents will also be discussed. The results were compared to off-line SPE which showed comparable results while offering significant time, labor and solvent savings. Further, the online method significantly reduced ion suppression in complex matrixes compared to off-line SPEand LVI. The method was also run on several different water matrixes including surface water, ground water and wastewater, always producing accurate and reproducible results.